Read the journal articles linked below, and answer the questions as thoroughly as you can. 

You may use whatever you resources you would like. If you work together with other students, you may submit one set of responses with everyone’s name on it. Submit your responses via the Course Content part of our myLelsey course page by May 4th. 

Bilingual neurons release glutamate and GABA

  1. What kind of article is this? Is it a peer-reviewed research paper or a magazine story or something else?
  2. The axons of the neurons described in the article originate in which region of the brain? In which brain region do they terminate?
  3. What evidence suggested that these neurons “have the ability to package glutamate and GABA into synaptic vesicles”?
  4. What does the term “bilingual neuron” in the title of the article refer to? What makes these neurons so interesting?
  5. What seems to be special about the synapses they form?

Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors

  1. Why did the authors obtain the crystal structure of acetylcholine binding protein (AChBP)? Why didn’t they obtain the structure of a nicotinic acetylcholine receptor?
  2. AChBP is described as a homopentamer. What does that mean?
  3. How many acetylcholine (ACh) binding sites does each AChBP have?

  4. Indicate the location of the ACh binding site(s) in panel a of figure 2, shown above.

  5. Is the nicotine binding site the same or different? 

  6. Is ACh or nicotine present in the actual crystals used to obtain the crystal structure of AChBP? If so, how does that molecule show up in the crystal structure? If not, how did the authors determine the location and nature of the ACh binding site(s)?